192 Wells/Kit Foot And Mouth Disease Virus Type O Antibody ELISA Kit

Foot and Mouth Disease Virus Type O Antibody ELISA Kit 192 Wells/Kit

1. Usage

Itis usedfor detection of FMD Type O antibody in serum of pig, bovine and goat. It is suitable for the detection of animal vaccine immune antibodies.

2. Principle

This kit adopts solid-phase competitive ELISA method, which is one of the designated methods of OIE for foot-and-mouth disease serological examination in international trade, and is also the reference method for the evaluation of the immune effect of foot-and-mouth disease vaccine inChina.
The ELISA plate was coated with FMDV-O VP1 recombinant protein antigen, and anti-VP1 protein monoclonal antibody was used as the enzyme label. Add the serum to be tested to the antigen-coated plate, and then add the enzyme-labeled monoclonal antibody. If there is FMDV-O-specific antibody in the serum to be tested, it will compete with the enzyme marker for the antigen in the coated plate, and the unbound components will be removed. Wash off, the color is light or no color after adding the substrate. Conversely, the color was darker after adding the substrate. Use a microplate reader to measure the OD450nm value, and determine the experimental results according to the calculation formula.

3. The kit components

4. Materials Required But Not Provided

1) Microplate Reader (single-wave length: 450 nm).
2) Precise micropipette (single-channel 1-100ul,0.5-10ul,multi-channel 30-300ul)
3) Constant temperature box orwater bath box.
4) Oscillator.
5) Disposable tips (10ul, 200ul)
6) Deionized water

5. Sample requirement

Try to use freshly collected serum samples; It cannot be used to detect samples with serious pollution or hemolysis; If the serum sample is turbid, take the supernatant after centrifugation for detection; The test sample can be stored at 2 ~ 8℃for 5 days. If it is stored for a long time, it needs to be transferred to - 20℃or lower temperature.

6. Preparation

1) BringELISA reagents to the room temperature(25±3℃) for at least 30 min to get best results. Microplate should return to room temperature and dry before open package.
2) Washingbuffer preparation: Dilute the10×concentrated washing buffer with deionized water at10 times. (for example: 10ml10×concentrated washing buffer + 90ml deionized water ).

7. Procedure

1) Loading: Add 50 µL of negative control serum (NC) and 50 µL of positive control serum (PC) to the control wells; first add 30 µL of sample diluent to the sample wells, and then add 20 µL of serum to each well; Add 50 μL of Enzyme conjugate to each well, shake gently to mix (do not spill), cover with sealing film, and incubate at 37°C for 30 minutes.
2) Wash the plate: discard the liquid in the well, add 300 μL of diluted washing buffer to each well, and wash 3 times. After the last wash, pat dry the ELISA plate on absorbent paper. It is strictly forbidden to dry the well plate between steps.
3) AddSubstrate: Add 50 μL of Substrate to each well, mix gently, cover with sealing film, and incubate at 37°C for 15 minutes in the dark.
4) Termination: Add 50 μL of stop solution to each well, shake for 10 seconds, mix well, and read after termination.
5) Reading: Measure the optical density value (OD value) at a wavelength of 450 nm with a microplate reader.

8. Results

1) For the assay to be valid, average OD value of Positive control＜0.4, average OD value of Negative control＞0.6.
2) Calculation method:ODNC Average value =(ODNC1＋ODNC2)/2
3) Sample S/N value calculation formula:S/N＝SampleOD value/ODNC Average value
4) Judgment standard: S/N＜0.5, judged as positive; S/N≥0.5, judged as negative.

9. Precautions and warnings for users

1) Please read the manual carefully before use.
2) Do not use expired reagents, and do not mix reagents from different batches.
3) The experimental garbage was sterilized by high pressure steam at 121℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes and then discarded.
4) Microplates removed from refrigeration should be equilibrated with moisture, dried at room temperature, and ready to be opened. Return unused MicroWell plates to dry foil bags and seal at 2-8°C. Unused liquid reagents should be covered and stored together with other components at 2-8°C away from light.
5) Samples and reagents should be added using a micropipette and frequently demonstrated for accuracy.
6) When adding washing buffer, it should be filled but not overflowed to avoid free enzyme or cross-contamination between wells.
7) The stop solution is corrosive, immediately rinse with plenty of water when it comes into contact with skin or clothes.

Specifications:96 wells×2.
Expiry date:12 months.
Storage:Store at 2~8℃, in the dark,no freezing.
Production Date:On outer-packing of the test kit.

For veterinary diagnostic use only

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: Usually by air, we will choose the best shipping method for you, and we can also ship according to the shipping method you require.