Shenzhen Wensidun Technology Co., Ltd.
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Porcine Epidemic Diarrhea (PED) Antibody ELISA Kit 192 Wells/Kit ISO9001
1. Usage
This kit is used to detect Porcine Epidemic Diarrhea (PED) antibody in porcine serum.
2. Notes
1) All reagents should be returned to room temperature (18~26℃) before use, and stored at 2-8℃ after use
2) Do not use expired kits. Do not mix reagents from different batches of kits.
3) Unused micropores should be sealed back in the bag and stored at 2-8°C.
4) Used materials should be disposed of harmlessly and in accordance with local, regional and national regulations.
5) Avoid exposing the substrate to strong light and avoid contact with oxidizing agents.
6) When the amount of serum samples is large, first dilute all serum samples on the serum dilution plate, and then transfer all the diluted serum samples to the reaction wells, so that the reaction time is the same.
7) The termination liquid is corrosive, if it splashes on the skin or clothes, it should be washed with plenty of water immediately.
8) Strictly follow the instructions for best results. All procedures including pipetting, timing, and washing must be accurate for accurate results.
3. The kit components
| 1 | PED antigen coated micro-plate | 96T X 2 | |
| 2 | Enzyme conjugate | 22ml | yellow lid |
| 3 | Sample diluent solution | 50ml | transparent lid |
| 4 | PED Negative control serum | 1.5ml | green lid |
| 5 | PED Positive control serum | 1.5ml | red lid |
| 6 | Substrate | 12ml *2 | orange lid |
| 7 | Stop solution | 12ml | blue lid |
| 8 | 20×concentrated washing buffer | 50ml | white lid |
| 9 | Adhesive Foil | 2 pieces | |
| 10 | Instruction | 1 piece | |
4. Preparation
1) Sample dilute: Dilute sample with the sample diluent at 40 times.(5ul serum + 195ul sample diluent), the diluted sample need to mix evenly to get better results. Negative control and positive control do not need dilute.
2) Washing solution preparation: Dilute the 20×concentrated washing buffer with deionized water or distilled water at 20 times. (10ml 20×concentrated washing buffer + 190ml deionized water ) It is normal if there is crystallization in the 20×concentrated washing buffer, put at 37℃until completely dissolved.
5. ELISA procedure
1) Take pre-coated microtiter strips (Can unseal for several time use as per sample quantity), add 100μL diluted serum to test well, meanwhile set 2 wells for Negative control, 2 wells for Positive control separately. Add 100 μL Negative/Positive control to its well. Shake softly, cover and incubate at 37℃ for 30 min.
2) Pour the liquid out of the wells, add diluted washing solution to each well fully, be static for 10s, pour out. Wash3 times, at last pat to dry on absorbent paper.
3) Add 100 μL Enzyme Conjugate to each well, and incubate at 37℃ for 30 min.
4) Repeat the step 2(washing). Remember pat to dry on absorbent paper at last.
5) Add 100 μL substrate to each well, mix properly,react for 10 min at 37℃ at dark.
6) Add 50 μL stop solution in each well, and measure the result within 10 min(shake evenly before read result, no spill).
7) Read OD value with ELISA reader at 450nm/630nm.
6. Results
For the assay to be valid, the positive control wells’ average OD value must be greater than or equal to 0.6, and the negative control wells’ average OD value is less than 0.1. Otherwise the test is invalid, need test again.
The result is judged by S/P value,
S/P=(Sample OD450/630- NCx(—))/( PCx(—)- NCx(—)), NCx(—) means Negative control’s average OD450/630 value(calculate as 0.05 when the value is less than 0.05), PCx(—) means Positive control’s average OD450/630 value
If S/P≥0.2, it is positive; less than 0.2, it is negative.
Specifications: 96*2 wells/kit.
Expiry date:12 months.
Storage: Storing at 2-8℃, in the dark.
Pathogenesis of porcine epidemic diarrhea virus
The virus enters the small intestine directly after oral and nasal infection. By immunofluorescence and electron microscopy, replication of the virus was carried out in the cytoplasm of the villous epithelium of the small intestine and colon. No virus proliferation was found in other organs. The proliferation of virus first causes damage to organelles, and then causes dysfunction of fine cells. The atrophy of intestinal villi leads to the reduction of absorption surface area, and the significant reduction of alkaline phosphatase content in small intestinal mucosa leads to nutrient absorption disorder, which is the main cause of diarrhea and belongs to osmotic diarrhea. Dehydration caused by severe diarrhea is the main cause of death of sick pigs.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.